|
KMID : 0361720040150030262
|
|
Korean Journal of perinatology
2004 Volume.15 No. 3 p.262 ~ p.267
|
|
|
Original Reports : Effects of magnesium sulfate on VEGF and Caspase-3 of HUVECs (Human umbilical vein endothelial cells)
|
|
Lee Gui-Se-Ra
Kim Sa-Jin
Kim So-Young
Lee Young
Park Sang-Hi
Shin Jong-Chul
Lew Young-Ok
Kim Soo-Pyung
|
|
|
Abstract
|
|
|
Objective: To determine the effects of magnesium sulfate on Caspase-3 and Vascular endothelial growth factor (VEGF) of human umbilical vein endothelial cells (HUVECs) under presence or absence of Tumor necrosis factor (TNF-α).
Methods: HUVECs were isolated from normal term umbilical cords and cultured in several physiologically
relevant concentrations of magnesium sulfate with or without exposure of TNF-α. The concentrations of VEGF and caspase-3 were estimated by colorimetric assay and ELISA assay, respectively.
Results: The concentration of VEGF in HUVECs significantly increased in the presence of TNF-α compared with in the absence of TNF-α. However, the concentration of VEGF did not show significant difference in several concentrations of magnesium sulfate concentrations with addition of TNF-α and it showed the lowest concentration under 4 mM and 8 mM of magnesium sulfate concentration without addition of TNF-α. The concentration of caspase-3 in HUVECs did not show statistically significant difference with the addition of TNF-α and magnesium sulfate.
Conclusion: TNF-α induce HUVECs to stimulate the VEGF, and magnesium sulfate might not have the effects on the expression of VEGF with addition of TNF-α. However, the concentration for treatment of magnesium sulfate inhibits the expression of VEGF without addition of TNF-α. Magnesium sulfate might not have an effect of the expression of caspase-3 with or without addition of TNF-α.
|
|
|
KEYWORD
|
|
|
VEGF, Caspase-3, HUVECs, Magnesium sulfate
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
  
|